New Test can Detect Bacteria Efficiently in UTI Diagnosis

New-Test-can-Detect-Bacteria-Efficiently-in-UTI-Diagnosis

A new enhanced test called enhanced quantitative urine culture developed by Loyola University Chicago, detects significantly more bacteria than the standard test.

The study was presented at a meeting of the American Society for Microbiology in New Orleans. Urinary tract infections (UTIs) are the most common type of infection in the urinary system. It affects bladder and urethra. Women are at higher risk of these infections and symptoms include a strong urge to urinate, a burning sensation when urinating, pelvic pain, and cloudy or discolored urine. Antibiotics are often used as the first-line treatment of UTI.

The current test uses a sample of urine, which is added to a substance (growth medium) that promotes the growth of bacteria in the urine. Two growth media are used and samples are incubated for 24 hours in room air. The new test, called enhanced quantitative urine culture (EQUC), uses a higher volume of urine. In addition to room air, samples are incubated in air containing a high concentration of carbon dioxide and in an anaerobic environment. Samples are incubated for 48 hours in three growth media.

A total of 150 urogynecologic patients were involved in the study and half of them reported to have symptoms of UTIs. Urine samples from the patients were subjected to both the standard culture and the EQUC tests. In 69 of the 75 women reporting UTI symptoms, the EQUC test detected one or more bacteria species, for a total of 110 species. Using the standard culture, only 50 percent of these bacteria species were identified. The standard culture identified most of the E. coli bacteria, but only 24 percent of the non E.coli bacteria.

According to Urinary Tract Infection Treatment Market report published by Coherent Market Insights, increasing prevalence of chronic kidney diseases and increasing use of urinary catheterization is leading to development of urinary infections which is increasing the demand for accurate diagnosis test for UTI. Above mentioned test can be use more efficiently than standard test. Loyola researchers are going to start a clinical trial to investigate whether using the EQUC method could improve the clinical care of women with UTIs. The trial will enroll 225 women who have UTI symptoms. Seventy five women will receive the standard culture plus EQUC and 150 women will receive the standard culture alone.

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